Hello,

I want to merge two SeuratObjects: h1 and h2, and do the integration.

https://satijalab.org/seurat/pancreas_integration_label_transfer.html

Thank you in advance for great help!

Best,

Yue

> a<-"hc_51_02.csv"
> a1<-data.frame(fread(a),check.names=FALSE, row.names=1)
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> h1<- CreateSeuratObject(counts = a1, project = "h1", min.cells = 3, min.features = 200)
> a<-"hc_52_02.csv"
> a1<-data.frame(fread(a),check.names=FALSE, row.names=1)
> h2<- CreateSeuratObject(counts = a1, project = "h2", min.cells = 3, min.features = 200)

> h1<-readRDS(file="h1.rds")
> h2<-readRDS(file="h2.rds")
> h12<-merge(h1,h2)
Warning message:
In CheckDuplicateCellNames(object.list = objects) :
Some cell names are duplicated across objects provided. Renaming to enforce unique cell names.
> pancreas <- CreateSeuratObject(h12, meta.data = meta.data02)
Error in as.vector(data) : 
no method for coercing this S4 class to a vector
In addition: Warning message:
In CreateSeuratObject(h12, meta.data = meta.data02) :
Some cells in meta.data not present in provided counts matrix.