Dear all, I have a HiFi sequencing (long read) and contig-level assembly of an insect (de novo whole genome assembly). Unfortunately, I don't have a …

Now I've got a database including 3000+ species of prokayotes, each of them has a taxid and a organim name. I wanna add a column …

I have downloaded 30 complete genome assemblies of a bacteria. I want to perform a 16s Rna genetic analysis.. I can extract the sequence manually...however...I …

Hello... Can anyone tell me how to maintain the consistency of the initial or raw data downloaded from ncbi site... Like the bacterial genus i …

I am doing the comparative analysis of a bacterial genus . Now, in the NCBI database, we have assemblies of this genus at different levels …

I'm studying a species where there is a reference genome that is assembled *only* at the scaffold level ("unplaced scaffolds"). See here https://www.ncbi.nlm.nih.gov/genome/annotation_euk/Geospiza_fortis/101/. My question …

Hi everyone, I am fairly new to bioinformatics and I am trying to wrap my head around genome sequencing and assembly. I need to analyze …

I often see this trend on GenBank assemblies where you have older chromosome level assemblies and then newer PacBio/illumina assemblies which are not. Are these …

I'm currently involved in the assembly (illumina data) of a few species with genome size 10-26Gb. I'm using the ABySS assembly software, mainly due to …

I am trying to build a database for metagenomic analysis have all the genomic.fna.gz files for bacteria and virus from ftp://ftp.ncbi.nlm.nih.gov/refseq/release/ release 77 which has …