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Issues analysing splitted fastq files with fastqc

Hello, I've downloaded some fastq files using fastq-dump --splt-files which results in 2 fastq files; the problem i have is that i don't know what's the meaning of each file and how i can analyse them with fastqc. can anyone help

fastqc

1 answer

FastQC only works on individual files and doesn't take into account paired end sequencing.

fastqc *fastq,gz

or

fastqc *fastq

Depending on the files you have, one of these should generate the standard quality control output for each of all the files you downloaded.

You need to QC paired-end data files independently. So there will be two QC files to look through once you do what @benformatics said above.

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