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Counting RNA-seq reads mapped to mRNAs (mouse)

Hi!

I need to count the number of reads that can be mapped to mouse mRNAs in a RNA-seq dataset. Much appreciated for any suggestions.

rna-seq

Use featurecounts or htseq-count. It needs a mouse gtf file too. You can download it from gencode or ucsc.

1 answer

If the reads are from a mixed-organism source, you can use software like BBSplit or Xenome to split reads into species specific files.

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