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Kallisto -s parameter ?

HI

I had the readlength of 100 bp, how do i calculate the -s parameter for running Kallisto. Suggestions please.

rna-seq

Another tool? Don't you want to solve the issues you have with your current analysis? I answered this ongoing problem here, https://support.bioconductor.org/p/132018/#132020 and suggest you stick with this pipeline and sort out the issues.

The standard deviation is something you have to derive experimentally, by loading the library on a Bioanalyzer.

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