Standard BLAST parameters
I'm very new to genome annotation and was wondering what the standard e-value and number of hits that are typically used in a BLAST search. I'm using BLAST on thousands of genes, to compare them to the reference genome for subsequent annotation. Any information or resources would be so helpful! Thank you
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Is there a reason you are not using a program like
makerto the initial annotation instead of using individual blast searches? There are no standard e-values and hits per se since every result is going to be need to looked at independently.