This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Cut off for dist.seqs step in MOTHUR

Hi community!!! I am analysing already submitted (in SRA) metagenome sequence data by MOTHUR software. here is some confusions

  1. I am seeing in SOP the cut off value for dist.seqs step is set to 0.03. In some other protocol I've also seen to set as 0.2. So, I'm confused, what value should I actually use for my study.

  2. Should I use the same value for cutoff in cluster(column=, count=, cutoff=) step also?

Can anyone please help me?

mothur 16s metagenomics
• 1,052 views • link
written by dpc dpc

0 answers

No answers yet.

Log in to answer this question.

More posts like this