Discarding duplicate reads from fastq file on the basis of random barcode
Say, I have used an oligo containing random barcode (Ns) for excluding the duplicated reads (PCR biased reads) Oligo: CCCTACACGACGCTCTTCCGATCTNNNNNNNNNNNNNNNNNNTGTAAAACGACGGCCAGT
So, how can I exclude/remove the duplicated reads from the fastq file? Thanks.
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naeem40thju •
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Where is this sequence supposed to appear in your reads? How is this sequence going to identify duplicate reads?
BTW: You have already received answers to do this in your last question here: How can I extract the sequencing reads containing a specific linker/tag?
Please don't post the same question in multiple threads.