How to prepare ranked-gene list for statistical enrichment test in PANTHER database
Hi all,
As per PANTHER manual, statistical enrichment test uses Mann-Whitney U Test (Wilcoxon Rank-Sum Test) to find enriched pathways. It requires a input file with two columns (gene id and expression values). It did not clearly specify what the expression values should be.
I have prepared a ranked gene-list by calculating expression scores (sign of logFC * log10pvalues) as mentioned in GSEA manual. But, GSEA uses modified kolmogorov-smirnov test.
My doubt is whether the expression score used for GSEA can be used for PANTHER enrichment also? Need expert opinion. Thank you!
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Can you link the tool you use? Doesn't PANTHER simply require a list of gene names?
I used this PANTHER link. Input requirements are mentioned here.
This was mentioned in the manual, under Statistical enrichment test.
The file format link did not open. So I calculated the expression score as per GSEA manual as mentioned in the question
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