Automatically change N for consensus sequence
I have thousands of fasta sequences, and many of them have N letters in the middle os alignment. I would like to use this sequences to test a tool, but i don't want just retire de N letters, but change it for consensus of my whole alignment. Any way to do it easily?
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It is nucleotide sequences by the way
can you please rephrase the question or give an example?
I want to replace the N letters with correspondent consense letter
Please define an invariable sequence with in the pattern. If every position is replaceable, it is not easy to replace the Ns.