Is FPM the only normalisation method recommended for CircRNAs?

Hi all,

I have a question. For circRNAs detected by RNA-seq, I've read various articles suggesting to use FPM (Fragments per million mapped reads) for normalisation of the raw counts. Is it wrong to use FPKM for circRNA normalisation - the difference being FPKM also corrects for the gene length.

Thanks and best!

rna-seq circrna normalisation

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updated 6.2 years ago by Kevin Blighe

• written 6.2 years ago by mimA •

1 answer

Hey,

Can you cite the sources? FPKM may correct for gene length, but it fails to correct for cross-sample differences.

Kevin

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written 6.2 years ago by Kevin Blighe

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