Hello,

I'm posting this hoping that you can help me understanding 3 unusual Tapestation Profiles. These profiles correspond to (DNA) libraries already indexed and cleaned for NGS. Attached are 6 images (2 of each profile). All the images correspond to Agilent D1000 ScreenTape.

I'm working with really degraded DNA so I'm never expecting to get a very high concentration (however, sometimes it's possible) or fragments larger than 300-400 bp.

Please let me know if you have ever encountered these type of profiles and/or if you have any suggestion for what might be causing this.

P.S. - I'm not entirely sure if the 1st and 2nd profiles are indeed different profiles. Also, before someone suggests that the profiles with higher peaks corresponds to too much DNA loaded on the tape, I'm not convinced that is the case, since these ones correspond to approx 20000 (pmol/l) and I have several samples with values up to 61800 (pmol/l) with a perfect profile.

Thanks in advance! Sofia

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