Meaning of "Truncated"
I'm trying to read a paper about in-vivo bacteriophage genome synthesis and I'm confused about the meaning of "truncated species" in the following sentence:
"However, there are several technical barriers to the synthesis of microbial chromosome-sized stretches of DNA that are hundreds of thousands to millions of nucleotides long, the most notable being the contamination of the oligonucleotides by truncated species."
What exactly is meant by "truncated" here and why does it make in-vivo synthesis of DNA more complicated? Thanks!
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truncatedspecies means those oligos that are not equal to the full-length product (e.g. bacteriophage genome). Since the oligos may differ in length fromfulltofull_length-n bp, they would be difficult to separate from desired product.In this context, species does not mean organism. It's probably that what confuses you.