How to get refFlat file which includes exon/codon lengths? Example in post

Hi All,

I have a list of 4000 genes (Transcript IDs/HUGO symbols) which I want to annotate with the following canonical information:

1       1190583 1190867 -       UBE2J2_NM_194315_exon8_544-828_182-276
1       1191425 1191505 -       UBE2J2_NM_194315_exon7_463-543_155-181
1       1192372 1192510 -       UBE2J2_NM_194315_exon6_324-462_108-154
1       1192588 1192690 -       UBE2J2_NM_194315_exon5_221-323_74-108
1       1198726 1198766 -       UBE2J2_NM_194315_exon4_180-220_60-74
1       1200163 1200210 -       UBE2J2_NM_194315_exon3_132-179_44-60
1       1203242 1203372 -       UBE2J2_NM_194315_exon2_1-131_1-44

I have tried downloading various formats of the refFlat database from UCSC (hg19), but I can't seem to get a format similar to this, or any way to convert their formats because I can't find the exon and codon/AA information(the 1-131 and 1-44 part from exon 2 for example) . I have all the exon start/stop info, and exon #, but not the other data.

Any help would be amazing.

Thank you!

genome assembly assembly

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written 6.3 years ago by blazer9131 •

Hello blazer9131!

It appears that your post has been cross-posted to another site: https://bioinformatics.stackexchange.com/questions/13026/how-to-get-refflat-file-which-includes-exon-codon-lengths-example-in-post

This is typically not recommended as it runs the risk of annoying people in both communities.

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written 6.3 years ago by ATpoint

Ah, I thought it would be nice to reachout to both communities to see if I was able to get an answer from one/other.

Will keep that in mind for the future :)

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written 6.3 years ago by blazer9131 •

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