This is a test version of Biostars. For the public version, visit https://www.biostars.org.
BLAT error while execution

Hi, I installed BLAT locally using :

conda install -c bioconda blat

Then I ran BLAT with the following command:

blat /path/to/hg19.fa /dev/null /dev/null -makeOoc=/path/to/hg19.fa.11.ooc -repMatch=1024

When I run my fasta file it gives me an error:

blat /home/user/hg19.fa /home/user/Desktop/unaligned.fasta -ooc= /home/user/hg19.fa.11.ooc 
Loaded 3137161264 letters in 93 sequences
mustOpen: Can't open  to read: No such file or directory

Kindly guide. Thanks.

blat error

That is odd. I just tried the command and was able to generate .ooc file from a single multi-fasta human genome file. I don't know why you are getting following line?

blat /home/user/hg19.fa /home/user/Desktop/unaligned.fasta -ooc= /home/user/hg19.fa.11.ooc

I just got the following:

Loading /path_to/genome.fa
Counting /path_to/genome.fa
Writing 11.ooc
Wrote 32617 overused 11-mers to 11.ooc
Done making 11.ooc

It was the same command as yours from original post. I assume the problem has been solved?

The output message has a whitespace there, is this whitespace also in your input command?

enter image description here

That may be a red herring unless the original command had a space. We have no way to know.

0 answers

No answers yet.

Log in to answer this question.