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Blastn query file with multiple sequences?

Hi, I am pretty new to using the blastn command on the command line, and I wanted a little help figuring things out. So I made my own blast database using the makeblastdb command, and now am using the blastn command. I have a fasta file with 3 different sequences in it. When I use the blastn command with this fasta file, does it blast all 3 sequences? I only want to blast one of the sequences in the fasta file, so what do I write in my command to specify which sequence in the file to use? Thanks!

sequence blast blastn fasta
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updated by AsoInfo AsoInfo • written by biogirl13 • biogirl13

1 answer

You can make a new file containing only that sequence and then BLAST against that db file.

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written by AsoInfo AsoInfo

Is there another way to do this without making a new file?

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written by biogirl13 • biogirl13

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