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Can I add the read counts to the re-sequenced read counts directly?

Hi there,

I have sequenced my samples twice. Can I add the first-time read counts to the second-time read counts directly? I just check the correlation and performed the PCA analysis. But I am not sure if I need to remove the batch effect?

Thanks for any answer and ideas.

rna-seq

1 answer

If you sequenced the exact same library twice, and used the same gene counting pipelines, yes, you can just combine them. Barring a qc problem, running the library over again doesn't add any technical error.

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