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STAR Generating Genome Indices error

$ STAR --runThreadN 8 --runMode genomeGenerate --genomeFastaFiles Rsolani.fa Feb 22 12:00:15 ..... started STAR run Feb 22 12:00:15 ... starting to generate Genome files WARNING: --genomeSAindexNbases 14 is too large for the genome size=715128832, which may cause seg-fault at the mapping step. Re-run genome generation with recommended --genomeSAindexNbases 13 Feb 22 12:00:17 then I changed the command , but still facing problems. $ STAR --runThreadN 8 --genomeSAindexNbases 13 --runMode genomeGenerate --genomeFastaFiles Rsolani.fa Feb 22 12:32:37 ..... started STAR run Feb 22 12:32:37 ... starting to generate Genome files

Genome_genomeGenerate.cpp:217:genomeGenerate: exiting because of OUTPUT FILE error: could not create output file ./GenomeDir//chrName.txt SOLUTION: check that the path exists and you have write permission for this file. Also check ulimit -n and increase it to allow more open files.

Feb 22 12:32:39 ...... FATAL ERROR, exiting what is this error? what should I do now?

rna-seq software error
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written by Balatheskulter • Balatheskulter

There is solution within "SOLUTION" message. You forgot to specify output directory in your command. Try to rerun the STAR with previous command and add:

--genomeDir output_folder
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written by ahaswer ahaswer

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