Hi,
Hope everyone is doing good. I am doing transcripyomic data analysis of control vs transgenic to find the difference in expression their pattern but as I started my study I found that FPKM value of internal control genes are higher than transgenics. I would really appretiate your help if anyone can guide how to normalize the data and then do analysis?
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written
by
asmita.bt14 •
1 answer
You can't do transcriptomics data analysis by comparing FPKM values of genes between samples. Transcriptomic data require careful normalisation and specialised statistical tests. Any one of a number of packages are available to perform differential analysis of transcriptomic data.
Please see vignettes for the following popular R packages:
DESeq2
EdgeR
Limma/Voom
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