location of integrated vector in the genome in CRISPR experiment
I have done a CRISPR experiment followed by whole genome sequencing and now I am trying to find the position of integrated vector in the genomes. is there any tool for this goal?
• 1,434 views
•
link
updated
by
lieven.sterck
•
written
by
Sara
•
written
by
Sara
1 answer
The approach offered by Brian Bushnell in this post Identification of the sequence insertion site in the genome has proven its value and efficiency several times for me when identifying Tdna insertion sites (I'm assuming it would work as well for CRISPR insertions).
The only requisite is that you know the sequence that has been inserted.
• 0 views
•
link
written
by
lieven.sterck
Log in to answer this question.
More posts like this
-
CRISPR-DAV: How to make a reference genome file
written by Sowmya •
-
extract pathogens from metaphlan3 results
written by serene.s •
-
INDELs frequency at the on-target site in CRISPR experiment
written by Sara
-
Getting the coordinates of targeted locations by sgRNAs
written by Sara
-
plot the number of variations within a certain window
written by Sara
-
read counts per chromosome arm
written by Sara
-
Software to find Molecular IDs
written by 112498262 •
-
How to get plasmid or costruct sequence from fastq file in c.elegans Whole-genome Sequencing?
written by GiV17 •
-
frequency of each nucleotide at each position in sequence
written by Sara
-
Visual Representation Of Multiple Genomes?
written by peris
what kind of data do you have?
your question is very brief, Please go through [[ Please read before posting a question ::: How To Ask A Good Question ]] and then consider editing your question.
Adding on this, you will notice that many of your previous questions went unanswered, and I think the lack of effort and details is a major factor for this. Is this lentiviral? What exactly did integrate into the genome and how?
@ATpoint: sorry. yes it is lentiviral
@lieven.sterck: I have done some edits. hope it is more informative.