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Species specific SNPs

Hi

We have performed GBS on a total of 17 species including subspecies, found a total of 14k SNPs to differentiate all the species. However, I would like to filter species-specific SNPs, for validation and their application. I have tested VCFtools (vcf-contrast) methods with vcf file as suggested but no method found suitable to filter SNPs.

Any suggestion from expert are most welcome

TIA

Seba

snp gbs phylogeny

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