How to produce a pdb file after introducing multiple point mutations?
i have introduced a mutations in an protein sequence based on literature review. i have their FASTA files, i have tried mutagenesis tool in pymol to produce multiple point mutations, then i used the same file for GROMACS molecular dynamics simulations to study its stability i am facing errors, i have tried SCWRL4 also, that output file also didnot work, Give me suggestion, like How to produce a pdb file after introducing multiple point mutations?
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Is this a protein with a known structure already?
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