Can I do a GO enrichment analysis with bacterial species abundance instead of expression values?
I have a large, but quite fragmented metagenome that has been binned into ASVs. From there we can work out the abundance of each ASV in a give sample. Can I use the abundance values as a proxy of gene expression values in the GO enrichment analysis (I don't have any RNA to get expression levels).
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You'll have to be clearer. What are you trying to achieve? You can make claims about DNA abundance (these samples have more microbes that contain these genes) but are they expressed? Who knows