Error in Cuffdiff
Dear friends,
I got following warning/error after running the cuffdiff. can anyone help to solve this error. Command is written above the warning/erro
Thanks
harshraj@harshraj-XPS-8930:~/ArabidopsisExpt$ cuffdiff -o Cuffdiff_out -u cuffmerge_out/merged.gtf control1_thout/accepted_hits.bam, control2_thout accepted_hits.bam, treated1_thout, accepted_hits.bam, treated2_thout/accepted_hits.bam
Warning: Could not connect to update server to verify current version. Please check at the Cufflinks website (http://cufflinks.cbcb.umd.edu).
terminate called after throwing an instance of 'std::__ios_failure'
what(): basic_filebuf::underflow error reading the file: iostream error
Aborted (core dumped)
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Looks like there is an error in your command line. These file paths don't seem to be correct
most likely you need
Files separated by comma need to be replicates. If they are all independent then no commas should be used.
I still have similar error as
Warning: Could not connect to update server to verify current version. Please check at the Cufflinks website (http://cufflinks.cbcb.umd.edu). terminate called after throwing an instance of 'std::__ios_failure' what(): basic_filebuf::underflow error reading the file: iostream error Aborted (core dumped)
So are those files actually in the paths you are indicating they are at in the command line above?
Please use
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