GENOME ASSEMBLY USING OXFORD NANOPORE SEQUENCING

Free University (FU) Berlin - 10 – 14 February 2020

This course will introduce the audience with a spectre of methods which are present in a usual assembly workflow, starting from raw data and finishing with a fully assembled genome. We will see how to obtain nucleotide sequences from raw signals, dive deeper into the most used assembly paradigm for long fragments, try out and compare several state-of-the-art assemblers, and at last, assess the quality of the obtained assembly with and without a reference genome. Structured over five days, this course consists of both theoretical and practical aspects which are intertwined through each day. The presented theoretical foundation will be applied on small bacterial datasets and visualized in order to better grasp the algorithms at hand.