Hellow everyone! I have met some questions about using Blast ring image generator. I installed the BRIG,and input some genome files and submitted them. But at last I can not get the ring map and only GC Skew and GC content exist. The system said ' C:\Program Files\NCBI\blast-2.10.0+\bin\makeblastdb -dbtype nucl -in C:\Users\laoliang1\Desktop\对比图\sequence.fasta BLAST Database creation error: mdb_env_open: 系统找不到指定的路径。' Because the blast software can not find the genome files, I can not get the genome ring I want. And I don't know how to solve the problem. I hope someone can help me. Thank you!