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When I use the function fpkmSCVPlot via cummeRbund, I get one of the lines is horizontal

When I analysis the differentially expressed gene , I used cummeRbund. I want to see the CV2 via the function fpkmSCVPlot, But I get one of the lines is horizontal, how can I interpret ?

I used command:

library(cummeRbund)
cuff<-readCufflinks("F:\\difference"
genes.scv<-fpkmSCVPlot(genes(cuff))
genes.scv
r rna-seq software error next-gen

Do you have replicates? Please show the plot.

thank you for your attention, I have two conditions ,and each condition has four replicates my picture

That link is broken or incomplete. Please follow the instructions linked by kristoffer.vittingseerup. Yu need the full link including the suffix (e.g. .png) and paste this into the image button field.

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