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How to get transcripts counts from STAR

Hello,

After successful runs of STAR, in the ReadsPerGene.out.tab file, I only have counts of Genes (ENSG/ENSMUSG) and I don't have counts of transcripts (ENST/ENSMUST), which I need.

My STAR index genome was obtained by reference genome from ENSEMBL and the .gtf annotation files were achieved from ENSEMBL.

This should be simple, but I can't figure it out. How can I achieve this?

Thanks

star rna-seq deseq2

1 answer

You can't get transcript counts directly from STAR. You have to output the BAM aligned to the transcriptome (option --quantMode TranscriptomeSAM) and then use RSEM, eXpress or Salmon to perform the quantification.

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