How to get transcripts counts from STAR
Hello,
After successful runs of STAR, in the ReadsPerGene.out.tab file, I only have counts of Genes (ENSG/ENSMUSG) and I don't have counts of transcripts (ENST/ENSMUST), which I need.
My STAR index genome was obtained by reference genome from ENSEMBL and the .gtf annotation files were achieved from ENSEMBL.
This should be simple, but I can't figure it out. How can I achieve this?
Thanks
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You can't get transcript counts directly from STAR. You have to output the BAM aligned to the transcriptome (option --quantMode TranscriptomeSAM) and then use RSEM, eXpress or Salmon to perform the quantification.
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