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Discrepancy in number of BLAST hits

Hi all,

I was just wondering if anyone here could shed light on a discrepancy I've noticed when retrieving and viewing blast results from NCBI.

The NCBI BLAST results page is displaying '243 results' - however, when I select all and download as an alignment hit table (CSV) and a FASTA of all the aligned sequences, I'm finding about a hundred additional entries appear in my exported results.

Does anyone know where these might be generated from?

alignment ncbi blast

1 answer

That should not be a discrepancy. Those hundred additional entries reported for each hit are high-scoring segment pairs (HSPs)

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