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Pairwise Alignment for Multiple Sequences in R

Hello y'all!

I have 15 protein sequences as fasta format in one file. I have to pairwise align them globally and locally then generate a distance score matrix 15x15 to construct dendrogram.

But when I do, i.e. A sequence is not aligning with itself and I get NA result. Moreover, AxB gives 12131 score but BxA gives NA. Thus R can not construct phylogenetic tree.

What should I do?

r pairwise alignment multiple protein

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