Hello,

I have merged three vcf files and translated the SNP calls using bcftools query and awk

$ bcftools query -f '%CHROM\t%POS\t%ID\t%REF\t%ALT[\t%TGT]\n' merge.vcf | awk -v FS="\t" -v OFS="\t" '{for(i=6;i<=NF;i++) {split($i, gt, "/"); if(gt[1]==".") $i="-"; else if(gt[1]==gt[2]) $i=gt[1]; else $i="N";} print }'
1   687 .   G   A   -   A   -
1   689 .   G   A   -   A   -
1   701 .   T   A   -   A   -
1   704 .   T   G   -   G   -
1   708 .   C   T,A T   -   A
1   6440    .   G   T   T   N   -

Here the heterozygous calls are called as N. I want to change the following heterozygous calls as IUPAC codes:

Code Base
R   A or G
Y   C or T
S   G or C
W   A or T
K   G or T
M   A or C
N   any base

I tried using:

bcftools query -f '%CHROM\t%POS\t%ID\t%REF\t%ALT[\t%TGT]\n' merge.vcf | awk -v FS="\t" -v OFS="\t" '{for(i=6;i<=NF;i++) {split($i, gt, "/"); if(gt[1]==".") $i="NA"; else if(gt[1]==gt[2]) $i=gt[1]; else if(gt[2]=="AG") $i="R";  else if(gt[2]=="CT") $i="Y"; else if(gt[2]=="GC") $i="S"; else if(gt[2]=="AT") $i="W"; else if(gt[2]=="GT") $i="K"; else if(gt[2]=="AC") $i="M"; else $i="N";} print }' > out.vcf

But it does not give IUPAC codes for the above heterozygous calls. Any suggestions/help is appreciated.

Thank you so much for your time!