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Assembling of 22 reads from D-LOOP failed

Hi, I wondered why the assembling of 11 PE reads from D-LOOP of wolf failed. I tried both with Sapdes and Uncycler. I attach the IGB tracks.

IGB screenshot

assembly

What do you mean by "failed"? What was the result? Two contigs? Three contigs? No contigs at all?

Best hypothesis you will get two contigs, as there is clearly a gap between ~630 to ~680bp.

There was no scaffold and no contigs. I used Spades embedded in Galaxy. Last rows of error log file:

=== Stack Trace === [0x5f9c32] [0x58ad81] [0x55d2c1] [0x408c19] [0x40123c] [0x87a73b] [0x407c01] Verification of expression 'cov_.size() > 10' failed in function 'void coverage_model::KMerCoverageModel::Fit()'. In file '/spades/src/common/modules/coverage_model/kmer_coverage_model.cpp' on line 187. Message 'Invalid kmer coverage histogram, make sure that the coverage is indeed uniform'. Verification of expression 'cov_.size() > 10' failed in function 'void coverage_model::KMerCoverageModel::Fit()'. In file '/spades/src/common/modules/coverage_model/kmer_coverage_model.cpp' on line 187. Message 'Invalid kmer coverage histogram, make sure that the coverage is indeed uniform'. spades-core: /spades/src/common/modules/coverage_model/kmer_coverage_model.cpp:187: void coverage_model::KMerCoverageModel::Fit(): Assertion `cov_.size()

10' failed.

== Error == system call for: "['/mnt/pulsar/server/dependencies/_conda/envs/__spades@3.12.0/share/spades-3.12.0-1/bin/spades-core', '/mnt/pulsar/files/staging/649831/working/K55/configs/config.info', '/mnt/pulsar/files/staging/649831/working/K55/configs/careful_mode.info']" finished abnormally, err code: -6

======= SPAdes pipeline finished abnormally and WITH WARNINGS!

=== Error correction and assembling warnings: * 0:00:01.344 8M / 1G WARN General (simplification.cpp : 479) The determined erroneous connection coverage threshold may be determined improperly ======= Warnings saved to /mnt/pulsar/files/staging/649831/working/warnings.log

=== ERRORs: * system call for: "['/mnt/pulsar/server/dependencies/_conda/envs/__spades@3.12.0/share/spades-3.12.0-1/bin/spades-core', '/mnt/pulsar/files/staging/649831/working/K55/configs/config.info', '/mnt/pulsar/files/staging/649831/working/K55/configs/careful_mode.info']" finished abnormally, err code: -6

In case you have troubles running SPAdes, you can write to spades.support@cab.spbu.ru or report an issue on our GitHub repository github.com/ablab/spades Please provide us with params.txt and spades.log files from the output directory.

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