Hi all,

I have installed PlasmidSeeker on my Linux MInt 19 computer and now am trying to run it for the first time.

I followed carefully this workflow

But when I run the command

perl plasmidseeker.pl -d db_w20/ -i TH19_1.filtered.fastq -b cereus-ATCC14579.fasta

The terminal replied

Loading database... Converting sample reads to k-mers... Can't exec "/home/fox/PlasmidSeeker/GenomeTester4/glistmaker": Permission denied at plasmidseeker.pl line 275. Can't exec "/home/fox/PlasmidSeeker/GenomeTester4/glistcompare": Permission denied at plasmidseeker.pl line 277. Finding coverage of bacterial isolate... Can't exec "/home/fox/PlasmidSeeker/GenomeTester4/glistmaker": Permission denied at plasmidseeker.pl line 283. Can't exec "/home/fox/PlasmidSeeker/GenomeTester4/glistcompare": Permission denied at plasmidseeker.pl line 152. Can't exec "/home/fox/PlasmidSeeker/GenomeTester4/glistcompare": Permission denied at plasmidseeker.pl line 153. Can't exec "/home/fox/PlasmidSeeker/GenomeTester4/glistcompare": Permission denied at plasmidseeker.pl line 159. Can't exec "/home/fox/PlasmidSeeker/GenomeTester4/glistcompare": Permission denied at plasmidseeker.pl line 160. Bacteria median coverage is 0 Bacteria median coverage is too low (less than 3). Higher coverage dataset is needed or use flag --ponly at plasmidseeker.pl line 287.

I have Googled for possible solutions, but nothing answered my problem. Could anybody help me?