This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Megahit Assembly - command line error

I am new to bioinformatics and I need help:((

I have downloaded megahit and working in the 'bin' directory

  1. I don't know where I am supposed to put my samples
  2. Even if I run the files with the specific directory from bin/Input/R1 and R2 nothing happens

    bin$ ./megahit -1 Input/Input-1_S6_L001_R1_001.fastq.gz -2 Input/Input-1_S6_L001_R2_001.fastq.gz -o A_Input

What is wring with my command line?

assembly fastq megahit

Do you get an error?

1 answer

This part of your command ./megahit means that you have a megahit executable in the present directory. If that's not the case, it won't work.

This part of your command Input/Input-1_S6_L001_R1_001.fastq.gz means that in that same directory you have a sub-directory called Input, and in that sub-directory a file called Input-1_S6_L001_R1_001.fastq.gz. Same for your other paired-end file.

These are possible errors that I can point out without actually knowing the error message. It would help if you post what that error message is.

Log in to answer this question.