Hi all,
I am facing an issue in choosing a correct fastq file from ENA, since there are two entries for same sample accession (ERS1042158). https://www.ebi.ac.uk/ena/data/view/ERS1042158
Experiment Accession is also same but the Run Accession is different. Can anyone please explain why there are two entries for 1 sample and what could be the possible difference in these two files? File size is also little different (87 GB vs 84 GB).
Thanks!
1 answer
There are sequencing (=same DNA pool, different lanes/flow cells) replicates. Download both, merge fastq files, then align. See here. This is normal for WGS especially in the pre-Novaseq era to split one samples over multiple lanes or flow cells as one lane/FC might not provide reads for the intended average coverage.
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