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Calculate the SNP effect on binding site and RNA fold for Bacteria

Dear all,

I would like to recalculate the effect of SNPs for the RNA fold and the binding site for bacteria genomes. I have the vcf file with SNPs comparing the isolates to the reference genome.

For the effect of SNP on RNA fold I have found:

and for the effect of SNP on binding site I have found:

The problem is the following, for effect on RNA fold I don't know which tool is mostly used and for effect on binding site the tool that I have found can't be used through command line at my local machine.

Grateful for any help :)

Damianos

snp bacteria rna_fold binding_site

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