This is a test version of Biostars. For the public version, visit https://www.biostars.org.
the output of trimmomatic software

hi everybody I use trimmomatic softwre for trimming and Iget 4output paired files(forward and reverse) unpaired (forward and reverse) i didn't inderstand the diffirence between the paired and unpaired files can you help me to inderstand please

next-gen

1 answer

See this for an explanation: Trimmomatic: What is the difference between paired and unpaired output files in paired-end mode

Paired files contain reads that survive trimming and are retained in both R1/R2 files. When one of the reads is discarded from R1/R2 its mate is put in "unpaired" file. Doing so keeps the order of surviving reads in paired files intact.

Log in to answer this question.