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Problem with QiaSeq panel
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13 months ago
Prague

Hi together, I have a problem with data analysis of QiaSeq panel. I use CLC workbench from Qiagen, but it is hard to use and not I do not understand many options. Is there alternative software? I can not find software that can work with UMIs. Any ideas? I am not good with bioinformatics. Sorry for bad english.

Cheers, Peter

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13 months ago
Deutschland

Hi Peter,

our genetic testing software 'Seamless NGS' offers an easy-to-use workflow for the analysis of Qiagen's QiaSeq panels.

Before mapping the reads, the common sequence and the UMIs are clipped away. After mapping, the UMIs sequences are properly used to correct for PCR clones and the single amplicon primer is clipped away to ensure correct and unbiased variant calling. The workflow was developed, tested and validated together with a trustworthy pathological laboratory at one of the oldest university hospitals in Germany. Having long-lasting experience in that field, together with these experts, ecSeq was able to get the most out of the QiaSeq panel.

If you are interested in our solution, please check www.seamless-ngs.com and/or write us a short email (support@seamless-ngs.com) to make an appointment for a personal live demo (online).

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Thanks. I look in it.

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13 months ago

Dear Mr. Peter Horbatz,

My name is Momchil Topalov and I am member of QIAGEN Bioinformatics Support team.

It is our understanding that you have used a QIAseq Panel and now you wish to have your data analyzed.

This can be achieved with CLC Genomics Workbench (https://www.qiagenbioinformatics.com/products/clc-genomics-workbench/) after installing the free Biomedical Genomics Analysis plugin (https://www.qiagenbioinformatics.com/plugins/biomedical-genomics-analysis).

From the Analyze QIAseq Panels guide you can easily import your data and navigate to the relevant panel and run the analysis workflow directly from the guide.

If you have additional questions or if you need further assistance, you can contact us directly on ts-bioinformatics@qiagen.com or through the contact support option in the Workbench.

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Thanks you for your kind help.

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