Hi everyone,

I'm trying to map two fastq files to M. fascicularis genome using the following command:

./subread-align -t 0 --sortReadsByCoordinates -T 5 -i ../../subread_index_normal/Macaca_fascicularis_5.0_genome -r ../../../../disk1/goekberk/SRR_files/SRR7529837_2.fastq -o BAM_files/SRR7529837_2.bam

Yet I'm receiving the following error:

        ==========     _____ _    _ ____  _____  ______          _____  
        =====         / ____| |  | |  _ \|  __ \|  ____|   /\   |  __ \ 
          =====      | (___ | |  | | |_) | |__) | |__     /  \  | |  | |
            ====      \___ \| |  | |  _ <|  _  /|  __|   / /\ \ | |  | |
              ====    ____) | |__| | |_) | | \ \| |____ / ____ \| |__| |
        ==========   |_____/ \____/|____/|_|  \_\______/_/    \_\_____/
      v1.6.4

//================================= setting ==================================\\
||                                                                            ||
|| Function      : Read alignment (RNA-Seq)                                   ||
|| Input file    : SRR7529837_2.fastq                                         ||
|| Output file   : SRR7529837_2.bam (BAM), Sorted                             ||
|| Index name    : Macaca_fascicularis_5.0_genome                             ||
||                                                                            ||
||                    ------------------------------------                    ||
||                                                                            ||
||                               Threads : 5                                  ||
||                          Phred offset : 33                                 ||
||                             Min votes : 3 / 10                             ||
||                        Max mismatches : 3                                  ||
||                      Max indel length : 5                                  ||
||            Report multi-mapping reads : no                                 ||
|| Max alignments per multi-mapping read : 1                                  ||
||                                                                            ||
\\============================================================================//

//================ Running (02-May-2019 16:13:20, pid=51088) =================\\
||                                                                            ||
|| The input file contains base space reads.                                  ||
|| The range of Phred scores observed in the data is [2,41]                   ||
*** Error in `./subread-align': malloc(): memory corruption: 0x0000000001f13390 ***
Aborted (core dumped)

I should also mention that each fastq file I'm dealing with is ~14 gb in size, so they are relatively large I guess. However, I'm not sure if the file size causes this error or is it something else. I tried to delete and re-download the fastq files in case they were corrupted, but received the same error.

I'd be more than happy if you could help me with this.

Best, Gökberk