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FastQC results interpretation

I am currently working on a project that aims at formulating a report that basically puts the output of FastQC in a more comprehensible form. So, instead of outputting simple graphs, a report is generated that includes possible interpretations of the graphs and suggestions regarding how to deal with the set of sequences in hand (i.e. trimming at specific position).

I am not a bioinformatics student, and my knowledge about sequencing and handling DNA or RNA-seq data is very limited.

Suggestions for sources that helps interpreting FastQC reports and relating it to the context in which the sequencing data will be used ( besides the FastQC manual of course) will be of much help. Also, any source about how the results of DNA-seq and RNA-seq might be different, or how could the sequencing data be used differently depending on the user's interest.

Thank you in advance.

dna-seq rna-seq sequencing shotgun fastqc
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updated by Kristoffer Vitting-Seerup Kristoffer Vitting-Seerup • written by Tasneem Fetian • Tasneem Fetian

3 answers

Try MULTIQC https://multiqc.info/

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written by urjaswita urjaswita

You can find the documentation for FASTQ including examples to help interpreting and illustrating good and bad examples here. Also not that the color in the overview in the left side indicate whether there is something that needs more attention (green = good, yellow = maybe, red = yes)

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written by Kristoffer Vitting-Seerup Kristoffer Vitting-Seerup

Try FASTQE

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written by 5heikki 5heikki

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