thank you very much !
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i don't know how to procedure A LOCAL Multiple DNA Sequence. should i use cluster omega? how should i set? thank you!
It depends on what are you looking for, there are a lot of aligners by command line or web-based, web-based is usually limited to specific maximum entries while the command line-based doesn't. You used the "assembly" tag in your question, be aware that alignment is not an assembly.
thank you very much !
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I would use MAFFT or MUSCLE for DNA, though ClustalO will work too. Simply feed the program your data (read the output of
—helpor the manual.Unless you have specific requirements, I would not alter any parameters.