Hi Shawn, thanks for your answer.
I know what GSEA is but I am not sure it can answer my questions for this particular hypothesis.
My question was more like "Do genes that are significantly up regulated within a specific FC interval show enrichment for some categories/ontologies/terms, compared to genes significantly up regulated within different FC intervals?".
Using random numbers and imagination for an example, let's say I have 70 genes that are significantly up regulated within a FC interval 1 to 1.5. Of these, 50 genes belong to ontology A. Within my universe/background (~ 15000 genes), there are 90 genes belonging to ontology A. Gene ontology enrichment testing of these 70 genes results in a very significant value for ontology A. Then I take all the significantly up regulated genes that have a FC > 1, which are, let's say, 1000 genes. Gene ontology enrichment testing using these 1000 genes results in no significance for ontology A.
I would like to understand if this makes sense from a biological perspective.