hi guys i try to use hisat2 aligning the fastq file to the index which i have built before, here is the command line: /home/wyf/miniconda3/bin/hisat2 …

Hi all, I am trying to index my bacterial genome using Hisat2 following the RNA-SEQ analysis workflow I wonder which genome file format I should …

Hi I'm trying to build the index for mouse genome using hisat2 but I'm getting the following error This is my command ``` hisat2-build -p …

Hi all I am using this command. hisat2 -p 8 -q -X ${READDIR}/hisat2_index_Cm_genome -1 ${READDIR}/tr.p.UE-2955-CMLib1_S88_L004_R1_001.fastq.gz -2 ${READDIR}/tr.p.UE-2955-CMLib1_S88_L004_R2_001.fastq.gz -S ${Output}/UE-2955-CMLib1.sam Error: Encountered internal HISAT2 exception (#1) …

Hi there, I'm trying to use hisat2-build to index my genome but it keeps coming back errors. I'm not sure how to fix it and …

Hi, I am completely new to this, any help will be greatly appreciated. After running hisat2, I get “Error, fewer reads in file specified with …

Hello, I am using HISAT2 for aligning transcripts to the reference genome. I have build the indexes first. And try the command as follows: hisat2-build …

Having some issue run hisat2. Keep getting error message. hisat2 -x ../yeast/yeast -U mini.fq -S mini.sam Could not locate a HISAT2 index corresponding to basename …

Hello, I am using HISAT2 for aligning transcripts to the reference genome. I run the following commands and then get errors: `Error: Could not open …

Hello I am trying to build my index to HISAT2 using the following command hisat2-build rn6_ercc/genome.1.ht2,genome.2.ht2,genome.3.ht2,genome.4.ht2,genome.5.ht2,genome.6.ht2,genome.7.ht2,genome.8.ht2 ht2_base I got the following errors >Error: could not …