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Can I conclude anything from MiSeq primer reads?
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11 months ago
francois • 10

I am sequencing with MiSeq loci I targeted with CRISPR-Cas9. I sometimes have many paired small primer reads (see pic attached), Can I conclude anything from them? They are definitely not random, I see more often at specific loci/in some samples. Can they be hint of large deletions?

sequencing • 117 views
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12 months ago
United States

Primer dimers are an indication that the library prep could be optimized; there's either an abundance of primers (or scarcity of PCR-able fragments of interest) or too many PCR cycles or all of it.

I don't think it would be appropriate to use them as evidence for anything related to your actual question of interest because they are quite frequently occurring artifacts.

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15 months ago
swbarnes2 5.7k
United States

Primer dimers don't tell you anything.

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