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Question: Infinium MethylationEPIC BeadChip probes with cross-hybridisation potential
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I would like to get the latest list of probes with cross-hybridization potential Infinium MethylationEPIC BeadChip. I want to download the file and use it to remove the probes before performing any analysis. Can anybody help?

ADD COMMENTlink 10 months ago Agaz Hussain Wani • 40 • updated 10 months ago Charles Warden 6.8k
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Check this paper: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909830/

ADD COMMENTlink 10 months ago Lemire • 390
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Thank you for your comment. I already go the data from that paper and was wondering if it is up to date.

ADD REPLYlink 10 months ago
Agaz Hussain Wani
• 40
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While this may not be the best direct answer to your question, I believe Zhou et al. 2016 and Pidsley et al. 2016 can give you possible reasons for filtering probes on the EPIC array.

Other than that, highly overlapping probes may both have cross-hybridization potential. However, I would generally prefer to focus on regions with multiple (preferably, at least 4 probes) within a CpG Island. So, if the probes don't overlap (but are both in the same promoter for a gene), I think that can give you some additional confidence. Also, unless the cross-hybridization is a huge problem, using methylation thresholds (like average beta > 0.7 in one group and average beta < 0.3 in the other group) may help. Those are the sort of things that I have implemented in the COHCAP package.

ADD COMMENTlink 10 months ago Charles Warden 6.8k

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