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Ballgown for visualization of gene expression

Dear all, Can we use Ballgown in order to establish (and visualize) gene expression from only one sample? So, it is not a differential analysis but rather to simply estimate which genes are the most expressed? If so, which value should one use as a covariate?

Thank you.

ballgown covariate

I think you cannot use 1 sample. Minimum 2 samples are required for this package

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