Hi,

I just got back some Pacbio isoseq data. So I sequenced my sample on two different cells. Both cells had the same sample on them. I combined the two cells and have already run them through ccs. I this is my first iso-seq project. I am not sure if I need to use lima to remove the adaptors? Since the sample was the same I don't believe it was barcoded? I didn't receive a fasta file with barcodes. So I am wondering if I even need to run my data with lima? or can you run lima without a fasta file with the bar codes?

Thanks!!