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BWA - [fread] Input/output error

[UPDATE] So sorry to update this so late, this turned out to be an error due to a hardware issue and was fixed when the hard disk was replaced.

Hello all,

I'm trying to align paired-end read files to the reference genome using the following command (bwa version: 0.7.17-r1188):

bwa mem $ref_genome_fasta Sample_R1_001.fastq.gz Sample_R2_001.fastq.gz

I get the following error after a few seconds:

[fread] Input/output error

[UPDATE] I was thinking it might be because of an issue with the input fastq.gz files but I checked them using fqtools -validate and they seem valid.

Has anyone encountered this issue or can help me to resolve it?

software error bwa alignment

You can use validateFiles from Kent utilities (linux version linked). Remember to chmod a+x validateFiles after you download the program.

$ validateFiles -type=fastq your_fastq.gz

Hello egeulgen!

We believe that this post does not fit the main topic of this site.

This turned out to be an error due to a hardware issue and was fixed when the hard disk was replaced.

For this reason we have closed your question. This allows us to keep the site focused on the topics that the community can help with.

If you disagree please tell us why in a reply below, we'll be happy to talk about it.

Cheers!

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