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scRNA-seq python tutorial

I'm looking for a python tutorial on scRNA-seq/RNS-seq data analysis. It will be helpful if someone could point towards the relevant resources.

scrna-seq python rna-seq

I guess you mean RNA-seq. I have to disappoint you. RNA-seq is most commonly analyzed in R using packages such as edgeR, DESeq2 or limma. Start with this tutorial on RNA-seq analysis with DESeq2. For scRNA-seq, search for Seurat tutorials. Can be found via google. Also in R.

2 answers

The scanpy tutorials might be a good start.

Fabian Theis' lab has recently published Lücken & Theis, Current best practices in single‐cell RNA‐seq analysis: a tutorial together with a nice github repository in which you can find an example case study Jupyter notebook written in Python. I can highly recommend this.

In case you would like to use it on your own data, you could head over to FASTGenomics. It is an online platform for single cell analytics and it offers a generalized version of the case study notebook, which might be applicable to your own dataset.

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