I'm looking for a python tutorial on scRNA-seq/RNS-seq data analysis. It will be helpful if someone could point towards the relevant resources.
2 answers
The scanpy tutorials might be a good start.
Fabian Theis' lab has recently published Lücken & Theis, Current best practices in single‐cell RNA‐seq analysis: a tutorial together with a nice github repository in which you can find an example case study Jupyter notebook written in Python. I can highly recommend this.
In case you would like to use it on your own data, you could head over to FASTGenomics. It is an online platform for single cell analytics and it offers a generalized version of the case study notebook, which might be applicable to your own dataset.
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I guess you mean RNA-seq. I have to disappoint you. RNA-seq is most commonly analyzed in
Rusing packages such asedgeR,DESeq2orlimma. Start with this tutorial on RNA-seq analysis withDESeq2. For scRNA-seq, search forSeurattutorials. Can be found via google. Also in R.Here is a nice compilation of all things scRNA by @Sean Davis: https://github.com/seandavi/awesome-single-cell While mostly
Rthere are somePythonthings in there.