CDF file for Cytoscan arrays
Hi there,
I'm trying to read some CEL files from Cytoscan HD arrays with the R package affy but I get this (annoying) error:
> u <- read.affybatch(filenames = list.files(pattern=".CEL$"))
> u
Error in getCdfInfo(object) :
Could not obtain CDF environment, problems encountered:
Specified environment does not contain CytoScanHD_Array
Library - package cytoscanhdarraycdf not installed
Bioconductor - cytoscanhdarraycdf not available
Además: Warning message:
missing cdf environment! in show(AffyBatch)
I've tried to build this CDF file through aroma package (from Affymetrix) and another error appears:
> cdf <- AffymetrixCdfFile$byChipType("2MO_(CytoScanHD_Array).CEL")
[2019-03-06 17:21:59] Exception: Failed to create AffymetrixCdfFile object. Could not locate an annotation data file for chip type '2MO_(CytoScanHD_Array).CEL' (without requiring any tags) and with filename extension 'cdf'.
at #03. byChipType.UnitAnnotationDataFile(static, ...)
- byChipType.UnitAnnotationDataFile() is in environment 'aroma.core'
at #02. byChipType(static, ...)
- byChipType() is in environment 'aroma.core'
- originating from '<text>'
at #01. AffymetrixCdfFile$byChipType("2MO_(CytoScanHD_Array).CEL")
- AffymetrixCdfFile$byChipType() is local of the calling function
Error: Failed to create AffymetrixCdfFile object. Could not locate an annotation data file for chip type '2MO_(CytoScanHD_Array).CEL' (without requiring any tags) and with filename extension 'cdf'.
Anyone could me where can I get this CDF file? Or any way to build it, please? Or to understand the error??
Thanks in advance. Regards.
• 3,164 views
•
link
0 answers
No answers yet.
Log in to answer this question.
The CDFs for these old Affymetrix arrays can be very difficult to find. A problem was that Affymetrix were purchased by ThermoFisher, and support for products was lost.
I have done a search and found these sources:
Thanks Kevin. I've downloaded the CDF file from the second link and I've move to the folder with the CELs but errors don't disappear... thanks anyway!
Strange - sorry about that. I would try to contact Affymetrix (Thermo Fisher). I think that there are still some remnants of the old support teams, whether they are officially now at Thermo is another question.